Moter region contains AP-1 binding sites [22,34]. Hence, we used ChIP-PCR assay to determine whether c-Jun/AP-1 was involved in LTA-regulated MMP-9 gene expression. We first designed a pair of primers for MMP-9 promoter (-597 to -318), containing an AP-1 binding site.Wang et al. Journal of Neuroinflammation 2010, 7:84 http://www.jneuroinflammation.com/content/7/1/Page 11 ofFigure 6 LTA induces MMP